| abstract |
(57) [Summary] (Modifications included) [PROBLEMS] Almost free from dyes, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin, and viruses, and not essentially glycated. Thus, the present invention provides a method for producing albumin having a relatively large amount of free thiol and a complete C-terminus. The method is characterized by passing albumin (preferably expressed and secreted by transformed yeast) through cation exchange followed by anion exchange chromatography in positive mode. Other steps, such as ultra filtration, Gel permeation chromatography, affinity chromatography (showing examples of binding albumin using a blue dye and examples of binding contaminants using an aminophenylboronic acid resin) may be used. Elution of albumin from a material having no specific affinity for albumin by a compound having affinity for albumin also discloses an example of removal of ammonium ions by a counter ion. |