| abstract |
(57) The present invention provides for the use of chemical or enzymatic reactions, including fluid manipulation, one or more temperature controlled incubation steps (including temperature cycling), and purification steps using molecular size discrimination, An automated device is provided that facilitates rapid processing of sub-milliliter samples. Exemplary applications include, but are not limited to, polymerase chain reaction (PCR), DNA sequencing applications, oligonucleotide chain reaction, ligase chain reaction (LCR), single base extension, exonuclease treatment, and oligonucleotide hybridization assays. Not done. |