| abstract |
(57) [Summary] Taxol, an anticancer drug, binds to β-tubulin in microtubules (MT) assembled in animal cells and causes cell cycle arrest, whereas the effect of taxol is It is different in fungi. For example, the ascomycete taxol-producing Pestalotiopsis microspora Ne 32 is taxol resistant (IC 50 > 11.7 M), and two oomycete species, Pythium ultimum and Phytophthora cinnamomi, are taxol sensitive (IC 50 0.1 μM). CDNAs encoding β-tubulin from P. microspora, P. ultimum and P. cinnamomi have been isolated. The deduced amino acid sequences of β-tubulin from P. microspora, P. ultimum and P. cinnamomi are: (1) a binding assay to detect taxol and taxol-like substances; (2) pharmacology of taxol on tumor samples (3) Designing drugs with taxol-like activity by utilizing information about the effect of particular residues on taxol binding; and (4) substituting the residues required for taxol binding It can be used to detect taxol and taxol-like activity by using the taxol-sensitive and taxol-resistant isogenic strains of P. ultimum thus constructed. |