http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2002537821-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2000-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2002537821-A |
| titleOfInvention | A method for large-scale cDNA cloning and sequencing by repeating subtraction |
| abstract | (57) [Summary] The present invention provides a cDNA sequencing method by repeating subtraction, comprising the following steps: (1) A step of preparing a high-quality cDNA original library using a tissue of an organism as a material (2) homogenizing the cDNA original library of step (1) according to the graded Cot value, thereby obtaining a cDNA homogenized library corresponding to the graded Cot value; (3) said step (4) using the sequenced cDNA clones to hybridize and subtract the homogenized cDNA library from the above step; Steps (3) and (4) are 1-50 0 times repeated step. Further, a preliminary subtraction hybridization step may be included in the method. By using this method, certain tissues or certain cDNAs can be efficiently and comprehensively sequenced. |
| priorityDate | 1999-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.