http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2002536021-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6489 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48 |
| filingDate | 2000-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2002536021-A |
| titleOfInvention | A novel metalloprotease of the neprilysin family |
| abstract | (57) Summary We describe an RT-PCR strategy that enables us to identify and clone members of the NEP-like family. Degenerate oligonucleotide primers corresponding to the consensus sequence placed on either side of the HEXXH consensus sequence for gincins were designed and used in RT-PCR using mouse and human testis cDNA. . A DNA fragment having a length predicted from the sequences of the above classes of enzymes was obtained. These DNA fragments were cloned and sequenced. Using the PCR strategy and the PCR fragment as a probe to screen a cDNA library, three gincin-like peptidases were identified in addition to the known members of the family. These cDNA sequences induce specific probes for Northern and in situ hybridization, probe human chromosomes to locate the gene, and establish potential associations with genetic diseases. Made it possible. In addition, these cDNA sequences were used to produce recombinant fusion proteins in Escherichia coli to produce specific antibodies. Finally, the cDNA sequence was cloned into a mammalian expression vector and transfected into various mammalian cell lines to generate active recombinant enzymes suitable for testing specific inhibitors. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009509564-A |
| priorityDate | 1999-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 313.