| abstract |
(57) [Summary]nThe present invention relates to a method for identifying the gene sequence of a potential vaccine antigen. Also encompassed are the gene sequences and the polypeptides they encode, as well as the use of such sequences to elicit a prophylactic immune response in animals. In particular, the present invention relates to the identification of the predominant antigenic gene sequence of mycoplasma, preferably Mycoplasma hyopneumoniae. In one aspect of the invention, there is provided a method of identifying an expressed protein translated from a nucleotide sequence in an expression vector, the method comprising the use of a marker co-expressed with the protein translated from the nucleotide sequence. Become. In a further aspect of the invention, there is provided a method of identifying a therapeutic antigen gene sequence encoding a therapeutic antigen protein for a disease from a sample of nucleotide sequences. Preferably, the marker is a poly His tag. |