| abstract |
(57) Abstract: treating human liver tissue to provide a substantially single cell suspension comprising one or more cell lineage progenitor and non-progenitor cells found in the human liver; To provide a debulked suspension enriched with progenitor cells displaying one or more markers associated with at least one of the cell lineages, the suspension may be enriched with a number of non-progenitor cells in the suspension. Subjecting to a substantially reduced debulking step, and from the debulked suspension, at least one marker associated with at least one hepatocyte lineage of the cell itself, its progeny, or a more mature form thereof Disclosed are methods of isolating and cryopreserving progenitor cells from human liver, comprising the step of selecting cells that express. These markers include CD14, CD34, CD38, CD45 and ICAM. Liver progenitor cells are 6~15μ diameter, a diploid, glycophorin A -, CD45 -, AFP +++ , characterized in that ALB + and is ICAM +, CD14 +, CD34 ++ , CD38 ++, It has different subpopulations in terms of CD117 + expression. These progenitor cell subpopulations have the expected characteristics for cells that are particularly useful in liver cell therapy and gene therapy, and for establishing bioartificial organs. |