| abstract |
(57) [Summary] The present invention provides a deletion type adenovirus vector. This inventive adenovirus vector carries IVa2, 100K, polymerase and / or terminal pre-protein sequences of the adenovirus genome. Adenoviruses may also contain other deletions, mutations or other mutations. In a particularly preferred embodiment, the adenoviral vector is deleted multiple times, ie, carries two or more deletions therein. The deletion adenoviruses of the present invention are "growth-defective" in that the virus cannot replicate and produce new virions in the absence of complementation function. Preferred adenovirus vectors of the invention carry a heterologous nucleotide sequence encoding a protein or peptide associated with a metabolic disorder, more preferably a lysosomal acid alpha-glucosidase. Further provided is a method for producing an inventive deletion adenovirus vector. Furthermore, there is provided a method of administering a deleted adenovirus vector to cells in vivo or in vitro. |