http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2002526123-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1999-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2002526123-A |
| titleOfInvention | Detection of nucleic acid polymorphism |
| abstract | (57) [Summary]nThe present invention relates to (a) a single strand of a DNA base sequence containing a mutation locus; (b) a hybridizable with the single strand (a) to form a double strand, and one of the mutations (C) specific for a double-stranded structure of a single strand (a) and a probe (b) forming a complex with the double strand; DNA complex is detected by monitoring the formation or dissociation of a complex consisting of a marker that forms a complex with the duplex and reacts independently when interacting within the duplex. The method comprises continuously measuring an output signal indicative of the interaction between the duplex and the marker and recording the conditions under which the change in the output signal of the reaction has occurred. Changes in the output signal are due to the formation or dissociation of the complex and therefore correlate with the strength of hybridization of the probe (b) to the single strand (a). This method, called Dynamic Allele Specific Hybridization (DASH), evaluates nucleotide differences in DNA sequences. Fluorescent markers that track changes in fluorescence resulting from complex denaturation or hybridization are suitable as markers (c) above. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2009130797-A1 |
| priorityDate | 1998-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.