| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2720-10022 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-245
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-21 |
| filingDate |
1999-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2002-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2002520060-A |
| titleOfInvention |
Cleavage of recombinant fusion protein produced by Caulobacter |
| abstract |
(57) [Summary]nThe present invention provides a method for cleaving target proteins from Caulobacter S-layer proteins under mild acid conditions. The fusion protein secreted by Caulobacter, including the target protein and the Caulobacter S-layer secretion signal, can be cleaved at the aspartic acid-proline dipeptide without solubilizing the fusion protein. The method can be performed with the fusion protein in an insoluble aggregate, which facilitates protein recovery. The present invention also provides a method for preparing a DNA construct for expressing the fusion protein, and a method for preparing the fusion protein. |
| priorityDate |
1998-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |