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filingDate 1997-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2002-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2002515974-A
titleOfInvention Rapid assay for neonatal infection
abstract (57) [Summary]nA rapid assay for infection in immunocompromised or immunocompromised patients such as newborns (neonatal infants) or immunocompromised patients (e.g., HIV or transplant patients) is used to diagnose at the time of initial assessment, so that antibiotic therapy and Avoiding sickbeds in the intensive care unit. This assay can be used to detect bacterial, viral, or mold colonies in the bloodstream, cerebrospinal fluid (CSF), or urinary tract. This method is particularly suitable for the diagnosis of sepsis. Polymorphonuclear leukocyte (PMN, neutrophil) CD11b (Mac-1, CR3) levels are measured by flow cytometry or laser scanning microscopy on low volume (0.1 ml) whole blood samples. Dual laser FACS identifies neutrophils with anti-CD15 fluorescent antibody conjugated with FITC and surface neutrophils CD11b marked with anti-CD11b antibody conjugated with PE. Spontaneous upregulation of CD11b is prevented by handling the sample at 4 ° C. or adding a stabilizing compound such as an anti-CD14 antibody or adenosine to the sample. The kit contains assay markers, such as CD11b-coated microspheres, as well as fluorescent anti-CD11b and anti-CD15 antibodies to assay the instrument used for analysis by diagnostic threshold.
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