http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2002512252-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1999-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2002512252-A |
| titleOfInvention | Reduction of endotoxin in nucleic acid purification |
| abstract | (57) [Summary]n The present invention provides a novel method for removing endotoxin contaminating nucleic acid, such as DNA, RNA, or a hybrid thereof, from the nucleic acid. Nucleic acid solutions that can be processed using the methods of the present invention include, but are not limited to, lysates of Gram-negative bacteria and nucleic acid solutions contaminated with endotoxins from external sources. The method of the present invention uses a silica-based material such as silica gel particles, magnetic silica particles, or diatomaceous earth to remove endotoxins from such solutions. In a preferred aspect of the method of the present invention, magnetic silica particles are used to isolate plasmid DNA from a lysate of Gram-negative bacteria transformed with the plasmid DNA. Application of this disclosed method produces nucleic acids useful for a variety of different practical applications, substantially free of endotoxin contamination. |
| priorityDate | 1998-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.