| abstract |
(57) [Summary]nThe present invention relates to the design, manufacture, and use of novel polypeptide bioactive agents. In particular, the invention is directed to targeting a polypeptide bioactive agent to a biological or chemical substrate via an engineered amino acid motif, while retaining or extending the biological activity by the polypeptide bioactive agent. About doing. The present invention provides a recombinant polypeptide bioactive factor, referred to as a matrix binding factor (MBF). In a polypeptide bioactive agent, the naturally occurring amino acid sequence of the factor is modified to make substitutions, deletions and / or additions of one or more amino acids that increase the affinity of the polypeptide bioactive agent for negatively charged surfaces. The polypeptide bioactive factor is preferably a growth factor that stimulates proliferation, differentiation, migration, cell activity, and more preferably a member of the growth factor insulin-like growth factor (IGF). The present invention also provides nucleic acid molecules encoding the polypeptide bioactive agents of the invention, methods of making the agents, and use of the agents in the treatment of various pathological conditions, use in tissue culture, surgical implantation and prostheses. Provided are compositions and methods for use in the present invention. |