http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2002507128-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1998-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2002507128-A |
| titleOfInvention | Additives for use in cycling probe reactions |
| abstract | (57) Abstract: A method for detecting a target nucleic acid molecule, comprising the following steps: (a) (i) a target nucleic acid molecule; (ii) a single-stranded nucleic acid probe containing a cleavable linkage; (iii) A) an enzyme capable of cleaving the probe portion of the double-stranded target-probe complex at the cleavable linkage; and (iv) a mixture comprising ribosomal proteins and / or spermine, wherein the target nucleic acid and the probe hybridize to each other. And reacting under conditions and for a time sufficient to allow formation of a double-stranded target-probe complex, followed by cleavage of the probe and cycling of the target to a new uncleaved probe. Releasing the one or more portions of the cleaved nucleic acid probe from the target-probe complex; and (b) the nucleic acid probe. Cut portions of the probe will determine whether it is produced, comprising thereby detecting the presence of the target nucleic acid, a method is provided. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018066713-A1 |
| priorityDate | 1997-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 187.