| abstract |
PROBLEM TO BE SOLVED: To provide a saccharide having a stronger enzyme activity on glycoprotein sugar chains than conventionally known α-mannosidase, acting near neutrality, and having a yeast mannose-1-phosphate bond. Provided is a novel α-mannosidase which can easily cleave mannose from a protein sugar chain. SOLUTION: α-mannosidase having an optimum pH of 6 to 9.5, and endo-α containing the α-mannosidase -Mannosidase, β-mannosidase, and endo- Provided is a fraction containing α-mannosidase, which is substantially free of β-N-acetylglucosaminidase. |