| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2002-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fcab6c4c53957755f48d399b89960c4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faa2dd5e79ef45b7880feccf81aa6c34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cff580034cf3bb2261384ba195a503f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c1035f648604bb9caedcd3821347957
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5acc0980f1ac61a851b88424cb7847c |
| publicationDate |
2002-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2002320493-A |
| titleOfInvention |
E. FIG. Methods for Cloning and Expression of BstYI Restriction Endonuclease and BstYI Methylase in COLI Method for purifying BstYI enzyme |
| abstract |
PROBLEM TO BE SOLVED: To provide a recombinant DNA encoding a BstYI restriction endonuclease and a BstYI methyltransferase, and an E. coli containing the recombinant DNA. The present invention also relates to the expression of the endonuclease and the methylase in E. coli cells and a purification method thereof. The present invention relates to a BstYI methylase gene and a BstYI methylase gene. A method of cloning stYI restriction endonuclease and expressing it in E. coli, and selecting Bacillu by methylase selection and inverse PCR amplification of adjacent DNA. Provided is a method for cloning BstYI restriction endonuclease from S. stearothermophilus in E. coli. After methylase selection, DNA A methylase gene having high homology to amino-methyltransferase (N4-cytosine methylase) was found in the library and was named BstYI methylase gene (bstYIM). |
| priorityDate |
2001-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |