| abstract |
(57) [Summary] (With correction) [Problem] To provide a luciferase derived from a decapoda (Decapoda), a polynucleotide encoding the same, and a method for producing a photoprotein thereof. SOLUTION: A secretory luciferase having a luminescence activity was isolated and purified from Rhododendron shrimp, and it was clarified that the secretory luciferase was composed of proteins having a molecular weight of 19 kDa and 35 kDa. The luciferase-constituting proteins 19 kDa and 35 kDa The gene encoding the protein was isolated, and the nucleotide sequence of the gene and the amino acid sequence encoded by the gene were determined. Furthermore, a recombinant vector containing the gene was prepared, and the protein was produced by a cell-free expression system using the vector or a protein expression system using a host transformed with the vector, for example, Escherichia coli or cultured animal cells. |