| abstract |
PROBLEM TO BE SOLVED: To provide a screening method for a substance capable of suppressing the absorption of a food allergen, an allergen absorption-suppressing substance, an allergen absorption-suppressing agent, and a food / drink or pharmaceutical containing these absorption-suppressing agents. To do. SOLUTION: Caco-2 cells derived from human colon cancer are cultured on a permeable membrane to form a small intestinal-like monolayer cell layer morphologically and functionally, and a sufficient tight junction is formed. Cells that are determined to be A test substance (fermented food or digested product) and a food allergen (such as ovalbumin) prepared in a buffer solution of pH 7.4 are added to the co-2 cells on the mucosal side and permeate from the mucosal side to the basement membrane side. The amount of food allergen per fixed area is measured for a certain period of time, and an allergen absorption inhibitor such as hexapeptide DKIHPE or tryptophan is screened. |