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publicationDate 2002-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2002253207-A
titleOfInvention Tissue cryopreservation method
abstract (57) [Summary] [PROBLEMS] To provide a method for cryopreserving cells in a vitrified liquid not containing serum without changing the cells without performing cell dispersion treatment or cell culture using digestive enzymes or the like. A tissue collected from a living body with a biopsy needle, Alternatively, a tissue collected from a corpse or a slaughterhouse just after death is cut into small pieces with scissors until a paste is formed, and the tissue is thoroughly washed with a phosphate buffer. Place the minced tissue in serum-free vitrification fluid, The suspension of minced tissue is placed in a cryopreservation container with an individual identification label, and sealed. After cooling this on liquid nitrogen, it is put into liquid nitrogen and stored in liquid nitrogen.
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