| abstract |
(57) [Problem] It is not necessary to add a different primer set for each target nucleic acid, and even if different primer sets are used, it is possible to obtain accurate results under the same reaction conditions for each primer set. To provide a method for measuring a nucleic acid. SOLUTION: (1) (a) a first primer consisting of a nucleic acid having a nucleotide sequence complementary to a 3 'terminal region of a template RNA, and (b) a nucleotide sequence identical to the 5' terminal region of the template RNA And a second primer having an RNA polymerase promoter sequence on the 5 'side on a primer-immobilized carrier immobilized on a solid phase so that the 3' end of each primer is free. A) amplifying the template RNA by bringing the primer-immobilized carrier into contact with the template RNA in the presence of a nucleoside triphosphate for forming a nucleic acid, a reverse transcriptase, and an RNA polymerase, and reacting the carrier; |