abstract |
(57) [Summary] [PROBLEMS] To provide a novel nucleic acid probe method and the like for easily and efficiently measuring or executing target nucleic acid measurement, polymorphism analysis, and real-time quantitative PCR. SOLUTION: When a nucleic acid probe is hybridized to a target nucleic acid, (1) a novel probe in which the fluorescence intensity of the hybridization reaction system decreases before and after the hybridization reaction, and (2) a fluorescence intensity of the hybridization reaction system. A fluorescent dye and a quencher substance are labeled so as to increase before and after the hybridization reaction, And a novel nucleic acid probe consisting of an oligonucleotide that does not form a stem loop between the base chain where the fluorescent dye is labeled and the position where the quencher substance is labeled, a measurement kit and a DNA chip using these, Nucleic acid measurement method, polymorphism analysis method, and real-time quantitative PCR method. |