| abstract |
(57) [Summary] [PROBLEMS] To reduce the reaction volume of a gene amplification reaction. SOLUTION: A plurality of channels 3 are formed on a surface of one substrate 1b constituting a reaction vessel chip 1. The dimensions of the channel 3 are such that the length Lc is 50 to 100 mm, the width Wc is 50 to 500 μm, and the depth D is 20 to 100 μm. The other substrate 1a is provided with a through hole as a reservoir 5 at a position corresponding to the end of the channel 3. The interval P between the reservoirs 5 and 5 arranged in a line in the width direction of the substrate 1a Is 1 / n of the pitch of the MTP format (n = 1 to 1) 0). The chip 1 is used in a state where both substrates 1a and 1b are overlapped and joined. At the time of use, the reaction solution is dispensed into the reservoir 5 at one end of each channel 3, The reaction solution is injected into the channel 3 by capillary action. By controlling the temperature of the chip 1 at a constant temperature or a predetermined temperature cycle, a gene amplification reaction is performed in the channel 3. |