| abstract |
(57) [Summary] [Problem] To culture a cell group derived from a specific cell, to observe culture while identifying cells to be interacted in the process of culturing the cell, and to keep the cell concentration constant Cultivating cells Spraying substances that interact with cells, such as signal substances, to specific cells in a cell group being cultured, and observing the difference between those cells and other cells Provide new technical means that enable Further, the present invention provides a new means for collecting only cells in a specific state and analyzing genes, expressed mRNAs and the like of the cells, or performing biochemical measurements. SOLUTION: The cell culture is provided with a cell culture part comprising a hole provided in a substrate shape, a semipermeable membrane covering an upper surface of the cell culture part, and a cell culture vessel having a culture solution exchange part provided on the semipermeable membrane. It is assumed that the apparatus includes means for supplying a cell culture solution to the container and microscopic means for long-term observation of cells in the cell culture section. |