| abstract |
PROBLEM TO BE SOLVED: To produce a novel (R) -2,3-butanediol dehydrogenase useful for producing ketones and alcohols, especially optically active vicinal diols. SOLUTION: It has been found that Pichia angusta produces a novel (R) -2,3-butanediol dehydrogenase with high activity and high stereoselectivity. Further, a DNA chain encoding this (R) -2,3-butanediol dehydrogenase was cloned, its base sequence was clarified, and the glycerol dehydrogenase was expressed using a heterologous microorganism. |