abstract |
(57) [Summary] The object of the present invention is to improve detection accuracy. Analysis can be performed in the order of μl / min, reproducible, highly accurate gradients can be obtained, inexpensive and easy to operate, and highly accurate measurement results can be obtained even when the flow rate is changed. When using a mass spectrometer as a detector, the entire amount of the target component can be introduced into the mass spectrometer, and when performing a mass spectrum measurement, the flow rate, pressure and gradient of the gradient are adjusted in synchronization with the measurement. It is an object of the present invention to provide a device and a measuring method. SOLUTION: In order to achieve the above object, a gradient liquid chromatogram measuring device 2 according to the present invention uses a liquid sending pump 4, 6 and a total volume of a gradient solvent used for at least one measurement. A liquid sending system 10 having a gradient solvent filling tube 8 that can be stored therein, and a sample injection mechanism 14 And a separation and detection system 20 including a separation column 16 and a detector 18. |