| abstract |
PROBLEM TO BE SOLVED: To provide a therapeutic agent for heart failure mainly comprising a cardiotonic drug, which has an action of enhancing the contractile force of the myocardium, but in severely ill patients, intracellular C The a 2+ concentration is not sufficiently reduced, so that sufficient relaxation cannot be obtained and an effective pump function cannot be obtained. In addition, since these drugs are accompanied by an increase in Ca 2+ influx from outside the cell, continuous Ca There was a problem that heart failure was further exacerbated by increasing 2+ concentration. SOLUTION: The present invention provides a mechanism for regulating Ca 2+ transport in the cardiac endoplasmic reticulum, which is a Ca 2+ storage site in cardiomyocytes (Ca 2+ pump- This is a method for screening for a drug having an action point on a phospholamban (phospholamban type), specifically, a drug screening method using inhibition of binding between a Ca 2+ pump and phospholamban as an index. |