http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001526525-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96466 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-567 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1997-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001526525-A |
| titleOfInvention | In vitro model for regulation of apoptosis and its study |
| abstract | (57) [Summary]nA cell-free system using the cytosol of normally growing cells is provided that reproduces the measurable characteristics of an apoptosis program. The apoptosis program is started by adding dATP, as a specific example, to 100,000 G supernatant of HeLa cells. A 15 kDa protein was obtained by fractionation of this cytosol, and it was identified as cytochrome c by determination of an absorption spectrum and an amino acid sequence. Cytochrome c is required for apoptosis in vitro. Apoptotic activity was lost by immunological removal of cytochrome c from the cytosol or by the addition of sucrose to stabilize mitochondria in the preparation of the cytosol. Addition of exogenous cytochrome c to the extract from which cytochrome c had been removed restored the apoptotic activity. Cells that undergo apoptosis in vivo have increased cytochrome c release into the cytosol, indicating that mitochondria are involved in apoptosis by releasing cytochrome c. |
| priorityDate | 1996-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 136.