http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001524317-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-126 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1998-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001524317-A |
| titleOfInvention | Methods for identifying and inhibiting functional nucleic acid molecules in cells |
| abstract | (57) [Summary]nTwo approaches are provided; the first provides a means to quickly and effectively identify essential and functional genes; and the second provides a biologically active nucleic acid molecule (ribozyme, EGS). , And antisense), which can be used to inactivate functional genes. In the first method, a library of EGS is prepared based on all possible known compositions. In a preferred embodiment, the EGS is 12-mer or 13-mer to the target bacterial RNAse for cleaving the substrate. This library is added to cells containing the gene to be screened (eg, E. coli). Cells in which EGS causes a loss of viability or other phenotype are identified. EGSs responsible for the loss of viability are analyzed and the resulting sequence information is used to identify genes within known genomic sequences. In a second method, a nucleotide molecule with optimal biological activity (eg, directing the cleavage of the gene of interest by RNase P) is used to generate two reporter genes (first in the phase with the gene of interest, and in the second). 2 are rapidly identified through the use of a vector containing the vector (either to prove the presence of the vector in the cell or as a control to aid in selection of cells containing the vector). Those cells in which the gene of interest is cleaved by the functional oligonucleotide molecule can then be identified by reference to reporter gene 1. The responsive functional oligonucleotide molecule is then isolated and characterized. These methods are powerful tools for identifying essential genes whose sequences are known only as part of the genome with unknown function, as well as identifying functional oligonucleotide molecules useful as diagnostic reagents and therapeutics. To provide the means for: |
| priorityDate | 1997-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 242.