http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001523098-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-10011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-11011 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 1998-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001523098-A |
| titleOfInvention | Method for the production of ASLV reverse transcriptase consisting of multiple subunits |
| abstract | (57) [Summary]nThe present invention generally relates to compositions and methods for reverse transcription of nucleic acid molecules, particularly messenger RNA molecules. In particular, the present invention relates to compositions comprising a mixture of polypeptides having reverse transcriptase (RT) activity, and to the use of these compositions or polypeptides, particularly at temperatures above about 55 ° C., for nucleic acid molecules ( In particular, methods for producing, amplifying, or sequencing cDNA molecules. The present invention also relates to nucleic acid molecules produced by these methods, vectors and host cells containing these nucleic acid molecules, and the use of such nucleic acid molecules to produce desired polypeptides. The present invention also provides for the production of Rous sarcoma virus (RSV) and avian myeloblastosis virus (AMV) RT, or other avian sarcoma-leukemia virus (ASLV) RT (α and / or β subunits thereof). Methods, isolated nucleic acid molecules encoding such RSV RT, AMV RT, or other ASLVRT subunits, vectors and host cells containing these isolated nucleic acid molecules, and produced by these methods For RSVRT, AMV RT, or other ASLV RT subunits. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, in particular heterodimeric RSV RT, AMV RT, or other ASLV RTs, which may be αβRT, ββRT, or αRT. (Especially baculovirus vectors) and host cells (especially insect cells and yeast cells) containing the nucleic acid molecules of the present invention, methods for producing these heterodimeric RTs, and heterodimeric RTs produced by these methods. . The invention also relates to a kit comprising a composition, polypeptide or RSV RT, AMV RT or other ASLV RT of the invention. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013165669-A |
| priorityDate | 1997-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 417.