| abstract |
(57) [Summary]nThe present invention relates to bleaching compositions containing protease variants. In one bleaching composition, 1,3,4,8,9,10,12,13,16,17,18,19,20,21,22,24,27,33,37,38 of Bacillus amyloliquefaciens subtilisin. , 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106 , 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166 , 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 09, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 The substitution of an amino acid residue by another natural amino acid residue at the amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin, together with the substitution of the amino acid residue by another natural amino acid residue at one or more amino acid residue positions Protease variant (the protease variant is in position 103 and 76) When substituting an amino acid residue at the corresponding position, Bacillus amyloliquefaciens subtilisin 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 210, 216, 217, 218, 222, 260, Amino acid residue substitutions at one or more amino acid residue positions other than the amino acid residue positions corresponding to positions 265 or 274), bleaching agents and one or more cleaning aids. In another bleaching composition, one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin undergo substitution of amino acid residues by other natural amino acid residues. A protease variant, a bleach and one or more cleaning aids. Methods for using the bleaching compositions are also provided. |