http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001514483-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6862 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1997-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001514483-A |
| titleOfInvention | Nucleic acid amplification method: Branching-elongation amplification method (RAM) |
| abstract | (57) [Summary]nAn improved method is provided that allows for rapid, sensitive and standardized detection of target nucleic acids from pathogenic microorganisms or viruses or normal or abnormal genes in a sample. The method comprises coating a target nucleic acid with a number of non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid (the probes are referred to as capture / amplification probes and amplification probes, respectively) and a ligand binding moiety. Hybridizing in the presence of paramagnetic beads. Binding of the ligand bound to one end of the capture / amplification probe and specific hybridization of a portion of the probe to an adjacent sequence in the target nucleic acid forms a complex of the target nucleic acid, probe and paramagnetic beads. The probes may be ligated together to form adjacent ligated amplification sequences bound to the beads, and the complex may be denatured to remove the target nucleic acid and unligated probe. Also, separate capture and amplification probes that form a continuous full-length or circular probe may be used, detected directly, or suitable for detection such as, for example, PCR, RAM or HSAM. It may be amplified by an amplification technique. Detection of the ligated amplification sequence, either directly or by subsequent amplification of the ligated amplification sequence, is indicative of the presence of the target nucleic acid in the sample. Including hybridization signal amplification and branch-extension amplification methods |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007517525-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8071750-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7167013-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019531747-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013539973-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007063807-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008527979-A |
| priorityDate | 1996-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 529.