| abstract |
(57) a method for amide transition peptide substrates with P 1 amino acid residue having a Abstract: positively charged side chains is provided. According to the present invention, in S 1 subsite, to replace at least one amino acid having a negatively charged side chains are modified carboxypeptidase Y. In addition, the modified carboxypeptidase Y can include substituted amino acid residues in the S 1 ', S 2 and / or S 3 subsites to accommodate a particular peptide substrate. |