http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001507935-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-89 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 |
| filingDate | 1997-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001507935-A |
| titleOfInvention | Methods and devices for microinjection of macromolecules into non-adherent cells |
| abstract | (57) [Summary]nThe present invention relates to a method for incorporating extracellular substances into cells present at non-adherent sites. In certain embodiments, a microinjection needle can be used to immobilize and use cells that are in a non-adherent position with an adhesive molecule or substance, or to support the desired injection of intracellular nucleic acids, such as nucleic acids. This is a method that can be used with both surfaces. Generally, non-adherent cells can be denatured by injecting a small amount of a nucleic acid sequence (DNA) at an effective site capable of giving an instruction to the chromosomal DNA ni of the cell. The invention also includes within the scope of modification of the cells according to the defined method, in particular undifferentiated cells, such as stem cells, genes or fragments which enable the replacement or normalization of abnormalities or deficiencies in the cells. It provides a method of gene therapy that can be made and used in patients by injection of cells. Normalized nucleic acids give rise to genetically normalized undifferentiated cells, providing an effective means for the treatment of subsequent physiological disorders. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009537137-A |
| priorityDate | 1996-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 397.