abstract |
(57) [Summary] The present invention relates to a method for preparing plasmid DNA using wet cell biomass, which comprises the following steps after resuspension of the biomass: alkali dissolution, high ionic strength acidification, removal of soluble substances, A method comprising reducing endotoxin and contaminating RNA, gel filtration chromatography, and conditioning. The invention also relates to a pharmaceutical composition containing the plasmid DNA, and its use for gene therapy. |