http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001501092-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1997-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2001-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001501092-A |
| titleOfInvention | DNA sequencing |
| abstract | (57) Abstract: The present invention is a method for identifying a base at a target position in a single-stranded sample DNA sequence, comprising preparing an extension primer that hybridizes to a sample DNA immediately adjacent to the target position, And the extension primer are subjected to a polymerase reaction in the presence of deoxynucleotides or dideoxynucleotides, whereby the deoxynucleotides or dideoxynucleotides are incorporated and pyrophosphate (PPi) only if they are complementary to the base at the target position. ) And enzymatically detecting PPi release, and adding different deoxynucleotides or dideoxynucleotides to separate aliquots of the sample-primer mixture or sequentially to the aliquots of the same sample-primer mixture, Perform the polymerase reaction and determine which deoxy A method for indicating whether nucleotides or dideoxynucleotides have been incorporated, wherein a PPi detection enzyme is included in the polymerase reaction step and acts as a substrate for the polymerase instead of deoxy or dideoxyadenosine triphosphate (ATP). A method is provided which uses dATP or a ddATP analog that is capable of acting as a substrate for the PPi detection enzyme, but is not capable of acting as a substrate. The method of the present invention advantageously allows for large-scale non-electrophoretic solid-phase DNA sequencing, thereby enabling a continuous determination of the progress of the polymerization reaction over time. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8853373-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009131176-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-5499037-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7915021-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2005517397-A |
| priorityDate | 1996-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 323.