| abstract |
(57) Summary An efficient method for producing recombinant AAV is described. In one embodiment, three vectors are introduced into a host cell. The first vector directs the expression of the cre recombinase, the second vector contains the promoter, a spacer sequence flanked by loxP sites and rep / cap, and the third vector is the AAV ITR ITR flanked by transfection. Includes minigenes containing genes and regulatory sequences. In another embodiment, two vectors are introduced into the host cell, where the host cell stably or inducibly expresses the cre recombinase and carries the other components of the system. |