| abstract |
PROBLEM TO BE SOLVED: To provide a protein having a junctionophilin activity necessary for constituting a junction membrane complex in a muscle cell such as skeletal muscle or a cell such as a neuron, a gene encoding the protein and a use thereof. To provide a method for diagnosing a disease associated with the expression or activity of a protein having a reduced juctophilin activity. SOLUTION: A hybridoma is prepared by a conventional method using membrane vesicles of rabbit skeletal muscle, and an immunoblot analysis of microsomal protein is performed using a monoclonal antibody obtained from the hybridoma. JP-1 is isolated and identified. Also, This protein rabbit JP-1 is composed of a large cytoplasmic region connecting the conjugated SR membrane and a C-terminal hydrophobic segment, and this cytoplasmic region (N-terminal side) can bind to the cell surface membrane, and a phospholipid as a membrane component And selective affinity. |