| abstract |
PROBLEM TO BE SOLVED: To provide a mutant acid phosphatase useful in a method for producing a nucleoside-5'-phosphate ester, a gene encoding the mutant acid phosphatase, a recombinant DNA containing the gene, and a recombinant DNA containing the same. Provide your own microorganisms. SOLUTION: A mutant acid phosphatase having reduced phosphatase activity and reduced phosphatase activity is preferably used for preparing nucleosides, polyphosphoric acid (salt), phenylphosphoric acid (salt) and carbamylphosphoric acid under conditions of pH 3.0 to 5.5. A nucleoside-5'-phosphate by producing a nucleoside-5'-phosphate by reacting with a phosphate donor selected from the group consisting of (salt); . |