http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001231599-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1010e3862914acdd087e73d76911ec6d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2000-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eab8ab3e628f22aee7c8854573f29304 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8959b33a766ba7b3db283dc312a2244e |
| publicationDate | 2001-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001231599-A |
| titleOfInvention | Methods for detecting genes with different expression changes |
| abstract | (57) [Summary] [PROBLEMS] The present invention can detect almost all genes that are theoretically expressed, and the results reflecting the expression level are simple, the number of operations is small, and the reproducibility and fidelity are improved. An improved differential display method (improved DD method) is provided which is excellent and low cost. SOLUTION: In the differential display method (DD method), a double-stranded cDNA prepared by reverse transcription of mRNA is cleaved with a restriction enzyme that generates a protruding end, and a base sequence of an immobilized portion of an adapter primer is changed. An improved feature characterized in that the nucleotide sequence completely matches or is complementary to the connecting terminal portion of the adapter oligonucleotide, or the difference in Tm value between the adapter primer and the oligo dT primer is 10 ° C. or less. A differential display method. The present invention also relates to kits and oligonucleotides used in these methods. |
| priorityDate | 2000-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.