Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcb146c68b67089013b4c7a614504254 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-975 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-808 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-968 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12007 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate |
2000-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1b23e5b280aa86bf4673a28a7c77141 |
publicationDate |
2001-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2001224398-A |
titleOfInvention |
Luciferase compositions and methods |
abstract |
(57) [Summary] (Corrected) [PROBLEMS] To provide an improved method and composition for analyzing a photo-induced rate derived from luciferase activity in a beetle luciferase-luciferin reaction. The present invention provides methods, compositions and test kits for assaying a sample in which beetle luciferase or ATP is present by using a beetle luciferase-luciferin reaction. Furthermore, a conjugate of coenzyme A, oxyluciferin in an excited state of the beetle luciferase and luciferase-luciferin reaction, and coenzyme A and luciferin (D-(-)-2- (6'-hydroxy- Also provided are thioesters of 2'-benzothiazolyl) -thiazoline-4-carboxylic acid). The figure shows that C When oA is present alone, it shows the emission curve of the field without any addition in the normal assay. And CoA, DTT In addition, when BSA was added, the total amount of generated light was 15 times or more that in the case where nothing was added. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7910087-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008113620-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009112199-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8216550-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010512759-A |
priorityDate |
1990-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |