http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001204469-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_438312df8a4ebd3fa8c445da07cb72aa |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N23-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
| filingDate | 2000-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f367653a0e7d7d0653d845ab5046861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1030f381025cabc9963a7098b953349a |
| publicationDate | 2001-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001204469-A |
| titleOfInvention | Crystallization method of nucleic acid-polypeptide complex, and nucleic acid and polypeptide used therein |
| abstract | PROBLEM TO BE SOLVED: To efficiently produce a large and homogeneous crystal capable of obtaining a good X-ray diffraction image, and to determine a phase for structural analysis for each test polypeptide. To provide a method for crystallizing a nucleic acid-polypeptide complex, which can eliminate the need for. SOLUTION: (1) a branching-forming polypeptide that binds to a nucleic acid and extends in a direction branching from the nucleic acid; (2) a test polypeptide to be examined for a binding state with the nucleic acid; A nucleic acid fragment comprising at least a region that binds to the branching polypeptide and a region that binds to the test polypeptide, both ends of which are cohesive ends and are substantially linked to other nucleic acid fragments. React with a nucleic acid fragment that can be extended in a rod-like manner, and link the plurality of nucleic acid fragments by adhering the cohesive ends of the nucleic acid fragment to each other, and in the branching direction by the branch-forming polypeptide. A crystal of a complex in which the branched polypeptide and the test polypeptide are bound to the linked nucleic acid fragments, thereby producing a crystal of a complex of the nucleic acid-polypeptide complex. Offered. |
| priorityDate | 2000-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.