| abstract |
PROBLEM TO BE SOLVED: To provide a novel and industrially advantageous reaction method for a reaction involving a mediator. SOLUTION: In a reaction method in which a mediator is activated by an enzyme or other mediator activating means to become an active mediator and acts on a substrate to perform a predetermined reaction, the active mediator is separately prepared outside the reaction system, Into a reaction system containing Preferably, the above-mentioned active mediator is prepared under mild conditions using an enzyme, and is introduced into a reaction system under highly efficient and severe conditions. Also preferably, a highly active or highly stable mediator with higher activity or higher stability is prepared by using any mediator activating means or the like, This is charged into the above reaction system. |