| abstract |
(57) Abstract: The present invention provides an improved method for somatic cell gene therapy utilizing enhanced gene transfer, a cell population for enhancing retrovirus-mediated DNA transfer into cells, and a novel method. It is intended to provide constructs, as well as their use. A method for increasing the efficiency of transduction of hematopoietic cells and other cells with a retrovirus involves infecting cells in the presence of fibronectin or a fibronectin fragment. As can be seen, fibronectin or fibronectin fragments significantly enhance retrovirus-mediated gene transfer into cells, especially hematopoietic cells, including committed progenitor cells and immature hematopoietic stem cells. |