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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88
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filingDate 1999-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3cd077f9edc1852f6094bc748956fa2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e369817a67209f014e4761c84990b9cd
publicationDate 2001-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2001161400-A
titleOfInvention Analysis of specific genes using immobilized DNA probes
abstract PROBLEM TO BE SOLVED: To specifically detect a specific DNA fragment of interest from a mixture of various types of DNA fragments, and to easily detect the presence or absence of a target DNA sequence, the presence or absence of a mutation, or the type of mutation. To provide NA analysis. SOLUTION: After a sample DNA fragment is denatured into a single strand, the sample DNA fragment is allowed to bind to a target DNA fragment in a column having a stationary phase on which an immobilized ligand is fixed. By contacting, only the target DNA fragment is specifically bound to the ligand, the other DNA fragments are washed away, and then the binding force between the ligand and the DNA fragment is reduced by gradually increasing the column temperature. D A DNA analysis method characterized by eluting an NA fragment from a column and analyzing the elution pattern. Further, an HLA typing method using the same is provided.
priorityDate 1999-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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