| abstract |
(57) [Summary] [PROBLEMS] The present invention provides (1) an immortal cell line obtained from a grouper fish and a method for establishing the cell line, (2) A method for mass-producing and purifying aquatic virus using an immortal cell line obtained from a grouper fish, (3) an anti-NNV antibody, a method for producing the anti-NNV antibody, and (4) a method for producing NNV. Disclosed are vaccines and methods for protecting fish from NNV infection. SOLUTION: The immortal cell line according to the present invention is obtained from a grouper fish, such as a two-stage ribonucleic acid virus (Birnaviridae) such as infectious pancreatic necrosis virus (IPNV), a formosa virus, and a formosa virus (EHVF). Herpesviridae, Reoviridae, such as hard shell clam reovirus (HCRV) And susceptibility to viruses belonging to the nodal virus family, such as neuronal necrosis virus (NNV). |