abstract |
PROBLEM TO BE SOLVED: To provide a simple and highly sensitive detection or quantification method of HCV and a detection or quantification reagent used therefor. An anti-HC labeled with alkaline phosphatase is disclosed. V-core protein antibody and HCV core antigen in a sample are subjected to an antigen-antibody reaction in an aqueous solution containing a high concentration of salt to form an immune complex, and alkaline phosphatase activity in the immune complex is measured. An immunoassay method for detecting or quantifying an HCV core antigen, and an alkaline phosphatase-labeled anti-HCV core protein antibody, A reagent for detecting or quantifying an HCV core antigen, comprising a salt at a concentration of 0.5M to a saturation concentration and a reagent for measuring alkaline phosphatase activity. |