abstract |
(57) [Problem] To provide a novel chitinase having a useful function and a method for producing the same. A chitinase having the following physicochemical properties: (1) Action: The β-1,4 bond of chitin is cleaved at random, and the N-acetylglucosamine 4 To form dimers and dimers, but N-acetylglucosamine Monomer does not produce, (2) Optimum pH: from 6.5 to 1 0.4, (3) stable pH: 7.0-9.0, (4) optimal temperature: 37 ° C, (5) working temperature range: 4-60 ° C, (6) Thermal stability: After heating at 40 ° C. and pH 8.0 for 30 minutes, an activity of 60% or more is maintained. A method for producing chitinase, comprising culturing a chitinase-producing bacterium belonging to the marine psychrotrophic genus Vibrio, which acts on chitin to produce an N-acetylglucosamine oligomer, and collecting chitinase from the culture. |