http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001112469-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aa908c0be49131bbf813d5a8635918ff |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
filingDate | 1999-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7add020456285d214a0ae7152126fa4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1d02a570ae9986af40f1680de3b0191 |
publicationDate | 2001-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2001112469-A |
titleOfInvention | Method for producing chib2 type chitinase |
abstract | (57) Abstract: belong A from soybean feedstock to PR-8 chitinase family, a chi b2 type chitinase a novel class, a simple column operation, a short time efficiently separated, a method of purifying To develop. SOLUTION: The defatted soybean powder is extracted with deionized water or acetate buffer, the protein fraction is separated from the obtained extract, the protein fraction is dialyzed, and then hydrophobic chromatography is performed. A fraction having chitinase activity is separated from the obtained eluate, and a method for producing a purified chi b2 type chitinase characterized by performing hydrophobic chromatography again, and the obtained purified chi b2 type chitinase, A method for producing a purified chi b2 type chitinase, comprising separating a lectin having and without a sugar chain by passing a binding lectin through a column bound to an insoluble carrier. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021508710-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11518781-B2 |
priorityDate | 1999-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.