http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2001099823-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8b9036c33bbe3f4ea428655b76200f7c |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-24 |
| filingDate | 2000-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0f4627a9e8b740e1b9fc2291fe5b1f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c5c208af31699543191837fc763a929 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c280c1a63a828240904e4ba16a03338 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc5ceabb4ecdd50303bada2fb441919a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec012c705dd106b9dbb416fc9fba3e58 |
| publicationDate | 2001-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2001099823-A |
| titleOfInvention | Method for producing trans-4-hydroxy-L-proline |
| abstract | (57) [Summary] [Object] To produce trans-4-hydroxy-L-proline useful as a pharmaceutical raw material or a food additive in an industrially advantageous manner. Also, it enables specific and sensitive automatic separation and analysis of trace amounts of optical isomers of prolines. The present invention relates to L-proline in an aqueous medium in the presence of an enzyme source that catalyzes a hydroxylation reaction of L-proline to trans-4-hydroxy-L-proline (t4HYP), ferrous ion and diketoglutaric acid. In a method for producing t4HYP, a method for analyzing L-proline 4-position hydroxylase useful for the production method, and a method for analyzing prolines using HPLC, characterized in that the eluate is converted to t4HYP, the eluate is supplied in a liquid sending line. Buffer solution containing a metal chelating agent, and reacting prolines in the eluate with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD) chloride to produce NBD-proline And a method for detecting and quantifying prolines. |
| priorityDate | 1993-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 289.