| abstract |
[PROBLEMS] To develop a method for isolating and purifying a nucleic acid such as a plasmid or genomic DNA from cells and other raw materials, and an apparatus for performing the method. A method and apparatus for isolating and purifying nucleic acids, including: a) removing cell debris and other particles by a filter layer having a reduced pore size in the direction of flow or in the direction of sample flow; b) The eluate is then treated with an anion exchanger in a low or high ionic strength buffer solution. The device is such that at least one of the filter layers 12, 20, 21 or 22 comprises a substantially cylindrical hollow body 1; Inside the inlet opening 7 than the layer 10 with anion exchange properties fixed between the two means 5, 6 or the layer 11 capable of binding nucleic acids in each solution of high ionic strength. It is located upstream when viewed from the direction. |